If preparing your own qc slides, 18 to 24hour cultures of known grampositive and gramnegative organisms are used. In most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear figure 1. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. A free powerpoint ppt presentation displayed as a flash slide show on id. This helps you give your presentation on structure of bacteria in a conference, a school lecture, a business proposal, in a webinar and business and professional representations the uploader spent hisher valuable time to. The gram stain virtual interactive bacteriology laboratory. This test can be used to confirm and determine gram stain reaction when if gram stain reagents arent available. Characterizing bacteria using gram staining lab 4l.
The circle farthest from the label contains a droplet of a gram negative escherichia coli derived from atcc 25922. Screening of the quality of the clinical specimens such as sputum that should contain many. We demonstrate the feasibility of using qpcr on dna extracted from vaginal gram stain slides to estimate the presence and relative abundance of specific bacterial pathogens. The system offers a large and robust database now accessible through the internetbased apiweb service. You will be asked about differential staining, grampositive and. Gram staining systems market global industry analysis. An intro to routine and special staining in histopathology. Controltest slides gram check test slides are provided with known gram positive and gram negative organism control blocks and six additional test blocks for your unknown specimens.
Our gram stain control slides have two wells containing a gram positive control organisms staph. Gram stain bacteria comparison set home science tools. The gram stain is the most important staining procedure in microbiology. Gram staining is an empirical method of differentiating bacterial species into two. Grampositive and gramnegative, making it a differential staining method. Gram stain synonyms, gram stain pronunciation, gram stain translation, english dictionary definition of gram stain. Basic classification of medically important bacteria nearly all clinically important bacteria can be detectedvisualized using gram staining method the only exceptions being those organisms. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from.
Winner of the standing ovation award for best powerpoint templates from presentations magazine. Grampositive bacteria thick layer of peptidoglycan90% of cell wall stains purple. Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain grams iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a. This video demonstrates the gram staining method for identifying unknown. Sop on gram staining objective to lay down the procedure for gram staining. Before staining, the specimen must be mounted and fixed on the slides, as previously done in the simple staining technique. Gram stain the previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria. Most bacteria can be differentiated by the gram reactions due to difference in their cell wall structure. Learn what it involves and when your doctor might order it. Csf gram stain from 2 day old infant 43 csf gram stain from 2 day old infant these are short gram positive bacilli.
Powerpoint is the worlds most popular presentation software which can let you create professional bacteria are everywhere powerpoint presentation easily and in no time. The gram stain is able to differentiate between gram positive and gram negative microorganisms ex vivo, as seen in fig. Caston of the clinical microbiology laboratory of the hospital of the university of pennsylvania. Gram staining lab 3 hans christian gram developed gram staining in 1844 the reagents primary stain. Primers were designed for amplification of total and speciesspecific bacterial dna based. In this procedure living, potentially pathogenic bacteria are smeared on the glass slide and allowed to air. Overheating of smears when heat fixation is used d. It includes introduction, history,principle, method of gram staining procedure slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Bacterial characteristics gram staining video khan. The gram stain 1 the gram stain 2 the gram stain is the most widely used staining procedure in bacteriology it is called a differential stain it differentiates between gram positive and gram negative bacteria. The most widely used staining procedure in microbiology is the gram stain, discovered by the danish scientist and physician hans christian joachim gram in 1884. The stain is retained because the cell wall of a grampositive bacteria is not as permeable as the cell wall of a gramnegative bacteria.
By colouring otherwise transparent tissue sections, these stains allow highly trained pathologists and researchers to view, under a microscope, tissue morphology structure or to look for the presence or prevalence of particular cell types, structures or even microorganisms such as bacteria. Based on differences in cell wall components, bacteria are categorized as either grampositive stains dark purple or gramnegative stains pink. Place slide on staining tray or hold with forceps above the sink. The gram stain procedure distinguishes between gram positive and gram negative groups by coloring these cells red or violet. Gram and acidfast stain control slides elitechgroup.
A grease free glass slide is taken and a circle is marked one side of the slide using a wax pencil or a glass marker pen. Corynebacterium diptheria seen in gram staining 35. The gram reaction reflects fundamental differences in the biochemical and structural properties of bacteria. Gramnegative bacteria thin layer of peptidoglycan10% of cell wall and high lipid content stains redpink. Although simple stains are useful, they do not reveal details about the bacteria other than morphology and arrangement.
Gram stain, a widely used microbiological staining technique that greatly aids in the identification and characterization of bacteria. The name gram staining comes from the danish bacteriology hans christian gram, who developed the microscopy technique. It was devised by a danish physician, hans christian gram, in 1884. Gram stain report introduction the purpose of this experiment is to determine the shape and gram stain of the bacteria under a microscope. An optimized staining technique for the detection of gram.
Gram negative pseudomonas aeruginosa bacteria pinkrods. This set contains the live bacteria cultures onlyorder our gram stain kit separately to perform the gram staining technique. The virtual microbiology classroom is organized by lecture into several topics. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. Ppt gram stain procedure powerpoint presentation free. The slides are intended to be part of a quality control program to monitor microbiological stains and staining techniques used in the. These online assessment tools will help you strengthen your understanding of the gram stain method. Rapid presumptive diagnosis of diseases such as bacterial meningitis. In most cases, the organisms cannot be identified by clinical presentation alone. Gram staining is a differential staining technique that differentiates bacteria into two groups. Pdf gram staining for the treatment of peritonsillar abscess.
A gram stain is a test used to learn if bacteria are present in your body fluids or tissue. Gram staining uses crystal violet to stain cell walls, iodine as a mordant, and. Bacteria that stain purple with the gram staining procedure are termed gram positive. Responsibility microbiologist or above of microbiology laboratory. Find powerpoint presentations and slides using the power of, find free presentations research about gram staining ppt. Gram stain definition of gram stain by the free dictionary. Scope this sop is applicable for gram staining in microbiology laboratory of pharmaceutical company name. A software was made that contains a certain algorithm and time intervals for the.
Therefore, when the decolorizer is applied to the smear. Detailsgram stain control slide provides two airdried and methanolfixed droplets within two etched circles. Gram negative gently stirred into a drop of 3% koh potassium hydroxide, the bacteria lyse break open, releasing their dna making the liquid viscous in 4560 secs. The circle nearest the label contains a droplet of gram positive staphylococcus aureus derived from atcc 25923. This helps you give your presentation on bacteria are everywhere in a conference, a school lecture, a business proposal, in a webinar and business and professional representations the uploader spent hisher valuable time to. Ppt staining of bacteria powerpoint presentation free. The culture will come with care instructions, but plan to use it within 12 days of arrival for best results. Ppt the gram stain powerpoint presentation free to. A rapid method for distinction between gramnegative and grampositive bacteria by means of a 3% solution of potassium hydroxide is tested on 71 grampositive and 55 gramnegative bacterial strains. Pericardial fluid gram stain is a method of staining a sample of fluid taken from. It is used to differentiate between gram positive organisms and gram negative organisms. Between 2005 and 2009, a total of 57 cases of peritonsillar. Gram and acidfast stain control slides elitechgroup usa.
Manufacturer or user prepared qc slides can be used. At the end of gram staining, gram positive bacteria appear violet or purple whereas gram negative bacteria appear pink. In this program, you may explore the following aspects of the gram stain and. Gram stains from either heat or methanolfixed slides stained with the new instrument were easy to interpret, and results were essentially the. View and download powerpoint presentations on gram staining ppt.
More likely, however, is that they are enterococci. Basic microscopy and viewing bacterial simple stain. Storing slides in a jar with 95% ethanol will ensure clean slides. Clinical utility of an automated instrument for gram. Selection of empirical antibiotics based on gram stain finding. Gramstain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. Hoffmannla roche ltd, lorne laboratories limited, lennox framework agreement, labema oy, axon lab ag and others. If you continue browsing the site, you agree to the use of cookies on this website. The reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be seen through the microscope. Selection of suitable culture media based on gram stain finding. Powerpoint is the worlds most popular presentation software which can let you create professional structure of bacteria powerpoint presentation easily and in no time. The staining technique, developed in the late 1700s by christian gram classifies the rigid cell walled bacteria into one of two groups based on whether they are able to resist the decolorizing action of an alcoholic. Gram staining principles gram staining is used to determine gram status to classify bacteria broadly. Gram staining sometimes called grams method is the method in which bacterial species are differentiated into two groups.
In addition to the gram reaction, the morphology and. Flood the slide with grams iodine and time for one. Flood slide with crystal or gentian violet 60 seconds. A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an. The gram stain is a type of differential stain that allows a microbiologist to. An ebook reader can be a software application for use on a computer such as microsofts free.
We first tested gram stained slides spiked with a mix of 108 cfuml of escherichia coli and 105 cfuml of lactobacillus acidophilus. However, nonspecific staining of damaged tissue is also apparent, which can ultimately overestimate bacterial colonization in tissues. Bacteria that retain the primary crystal violet stain that is first applied as part of the staining process are classified as grampositive bacteria. Gram stained control slides must be verified before examining and reporting patient smears. To examine whether gram staining can influence the choice of antibiotic for the treatment of peritonsillar abscess. Gram positive bacteria dont lyse in 3% koh so dna is released. Flood the surface of the slide with crystal violet stain and let sit for one minute. Learn for free about math, art, computer programming, economics, physics, chemistry, biology, medicine, finance, history, and more. The gram stain is the most frequently used stain in a clinical microbiology laboratory and is usually the first step in identifying bacteria. In 1884, hans christian joachim gram improved a staining method with the. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. The gram stain control slides are prepared from standard quality control organisms. Rao md for benefit of medical and paramedical professionals. Khan academy is a nonprofit with the mission of providing a free, worldclass education for anyone, anywhere.